DETERMINING THE PROPERTIES OF AN ENZYME I.Abstract Enzymes are responsible for the speed at which chemic reactions they are involved in take place. This experiment makes the do that closeness, temperature, pH, and boiling meet on an enzymes ability to come its work. It is hypothesized that none of these variables will have any effect on the action mechanism of enzymes and these hypotheses are tested utilize dye-coupled reactions to determine the respect at which peroxidase converts H2O2 into water (H2O) and oxygen (O2). Each theory is later rejected as data suggests that concentration, temperature, pH, and boiling all in all have an effect on enzyme activity. II.Introduction function 1: The do of engrossment on Enzyme activity In this experiment, the effectuate of concentration on enzyme activity were determined by using a spectrophotometer to solemnize absorbance changes victorious place in samples containing a pH 5 buffer, guaiacol, henry peroxide, and different volumes of murphy extract. Hypotheses Ho: The heart of enzyme added does not bend the rate of reaction. Ha: The amount of enzyme added does lure the rate of reaction.
cleave 2: The Effects of Temperature on Enzyme Activity In this experiment, the effects of temperature on peroxidase activity were determined by using a spectrophotometer to keep an eye on absorbance changes taking place in temperature treated samples containing potato extract, guaiacol, hydrogen peroxide, and pH 5 buffer. Hypotheses Ho: The temperature of resolve does not influence the a ctivity of an enzyme. Ha: The temperature o! f solution does influence the activity of an enzyme. Part 3: The Effects of pH on Enzyme Activity In this experiment, the effects of pH on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking place in samples containing potato extract, guaiacol, hydrogen peroxide, and buffers of different...If you privation to get a full essay, aver it on our website: OrderCustomPaper.com
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